Coding

Part:BBa_K2768004:Design

Designed by: Wenfei Yu   Group: iGEM18_USTC   (2018-09-18)


GFP with SsrA tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644


Design Notes

We want GFP to be degraded fast, so that it can disappear quickly when the expression is inhibited.


Source

Sequence from BBa_E0040 and BBa_M0052.

References